Composite

Part:BBa_K1124101

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

clock device (plux/tet) (+luxI, +tetR, +aiiA, ++sRNA, +amilGFP)

The intermediate construct for Multicellular Analog Clock. This BBa_K1124101 contains "gear" and "clock hand" (wiki‚Ìsystem‚ւ̃Šƒ“ƒN). 

 LuxI and LuxR forms the positive feedback loop of AHL production.
 TetR, AiiA, and sRNAs are the suppressors for negative feedback loops.
 mCherry is the red reporter protein for visualization of the excitation wave (gclock handh).
 amilGFP is the yellow reporter protein expressed constitutively as an internal standard.

You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3873
    Illegal NheI site found at 3896
    Illegal NheI site found at 4860
    Illegal NheI site found at 4883
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 326
    Illegal BsaI.rc site found at 654


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Categories
Parameters
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